Superficial cells
Epithelial cells exfoliated from the vagina during estrus. They are also called cornified or anucleated cells and are the largest of the epithelial cells present during estrus. The nuclei of these cells appear faded or absent. These cells stain lightly, may appear multi-layered, and with folded borders. If 80-90% of the cells in the smear are superficial cells that can be used as an indication to start breeding. Epithelial cells from the vagina which are also called cornified or anucleated cellsa and are the largest of the epithelial cells present during estrus. If 80-90% of the cells in the smear are superficial cells that can be used as an indication to start breeding.